phyloBARCODER (ver.1.0)



Phylogenetic tree:

Alignment of blast hits:
168 sites (1) were used for tree reconstruction by excluding 12 sites (0).
                                                                           1
Used4treeSearch                                                            001111111111111111111111111111111111011111111111111111111111111111111111111111111111111111
AP009617.1_SRRNA_Engraulidae_Engraulinae_Amazonsprattus-scintilla_BlueHit  GCCACCGCGGTTATACGAGAGACCCTAGTTGATTGA-AGCGGCGTAAAGAGTGGTTATGGAATTATAT-TTTTAAAGCAGAAAGCCTCTC
MH613715.1_SRRNA_Engraulidae_Engraulinae_Engraulis-mordax_BlueHit          GCCACCGCGGTTATACGAGAGACCCTAGTTGATTGA-AGCGGCGTAAAGAGTGGTTATGGAGTTTTCCCCTTTAAAGCAGAAAACCTCTC
LC557053.1_SRRNA_Engraulidae_Engraulinae_Engraulis-japonicus_BlueHit       --CACCGCGGTTATACGAGAGACCCTAGTTGATTGA-AGCGGCGTAAAGAGTGGTTATGGAATTTTCTACCCTAAAGCAGAAAACCTCTC
DQ912050.1_SRRNA_Engraulidae_Engraulinae_Engraulis-eurystole_BlueHit       ---------------------------------------CGGCGTAAAGAGTGGTTATGGAATTTTTTACCCTAAAGCAGAAAACCTCTC
MT410920.1_SRRNA_Engraulidae_Engraulinae_Engraulis-encrasicolus_BlueHit    GCCACCGCGGTTATACGAGAGACCCTAGTTGATTGA-AGCGGCGTAAAGAGTGGTTATGGAATTTTTTACCCTAAAGCAGAAAACCTCTC
AF417342.1_SRRNA_Engraulidae_Engraulinae_Engraulis-japonicus_BlueHit       GCCACCGCGGTTATACGAGAGACCCTAGTTGATTGA-AGCGGCGTAAAGAGTGGTTATGGAATTTTCTACCCTAAAGCAGAAAACCTCTC
USERDB14368_gb_095_027_Engraulis_japonicus_BlueHit                         --CACCGCGGTTATACGAGAGACCCTAGTTGATTGA-AGCGGCGTAAAGAGTGGTTATGGAATTTTCTACCCTAAAGCAGAAAACCTCTC
LC604998.1_SRRNA_Engraulidae_Engraulinae_Engraulis-japonicus_BlueHit       GCCACCGCGGTTATACGAGAGACCCTAGTTGATTGA-AGCGGCGTAAAGAGTGGTTATGGAATTTTCTACCCTAAAGCAGAAAACCTCTC
ANON2_OTU_321_B06-0m_BlueHit                                               GCCACCGCGGTTATACGAGAGACCCTAGTTGATTGA-AGCGGCGTAAAGAGTGGTTATGGAATTTTCTACCCTAAAGCAGAAAACCTCTC
USERDB1672_gb_095_001_Engraulis_japonicus_BlueHit                          --CACCGCGGTTATACGAGAGACCCTAGTTGATTGA-AGCGGCGTAAAGAGTGGTTATGGAATTTTCTACCCTAAAGCAGAAAACCTCTC
USERDB1697_gb_AB040676_Engraulis_japonicus_BlueHit                         --CACCGCGGTTATACGAGAGACCCTAGTTGATTGA-AGCGGCGTAAAGAGTGGTTATGGAATTTTCTACCCTAAAGCAGAAAACCTCTC
LC519335.1_SRRNA_Engraulidae_Engraulinae_Engraulis-japonicus_BlueHit       --CACCGCGGTTATACGAGAGACCCTAGTTGATTGA-AGCGGCGTAAAGAGTGGTTATGGAATTTTCTACCCTAAAGCAGAAAACCTCTC
ANON4_OTU_032_B06-0m_BlueHit                                               GCCACCGCGGTTATACGAGAGACCCTAGTTGATTGA-AGCGGCGTAAAGAGTGGTTATGGAATTTTCTACCCTAAAGCAGAAAACCTCTC
USERDB1698_gb_AF417342_Engraulis_japonicus_BlueHit                         --CACCGCGGTTATACGAGAGACCCTAGTTGATTGA-AGCGGCGTAAAGAGTGGTTATGGAATTTTCTACCCTAAAGCAGAAAACCTCTC
USERDB1716_gb_KF765500_Engraulis_japonicus_BlueHit                         --CACCGCGGTTATACGAGAGACCCTAGTTGATTGA-AGCGGCGTAAAGAGTGGTTATGGAATTTTCTACCCTAAAGCAGAAAACCTCTC
KF765500.2_SRRNA_Engraulidae_Engraulinae_Engraulis-japonicus_BlueHit       GCCACCGCGGTTATACGAGAGACCCTAGTTGATTGA-AGCGGCGTAAAGAGTGGTTATGGAATTTTCTACCCTAAAGCAGAAAACCTCTC
ANON12_OTU_157_B06-0m_BlueHit                                              GCCAACGCGGTTATACGAGAGACCCTAGTTGATTGA-AGCGGCGTAAAGAGTGGTTATGGAATTTTCTACCCTAAAGCAGAAAACCTCTC
ANON11_OTU_040_B06-0m_BlueHit                                              -CCACCGAGGTTATACGAGGGACCCTAGTTGATTTA-ATCGGCGTAAAGAGTGGTTATGGAGAATAAGAAACTAAAGCCGAAGACCTCTT
ANON3_OTU_007_B06-0m_BlueHit                                               GCCACCGCGGTTATACGAGGGACCCTAGTTGATTTA-ATCGGCGTAAAGAGTGGTTATGGAGAATAAGAAACTAAAGCCGAAGACCTCTT
ANON10_OTU_391_B06-0m_90%                                                  --CACCGCGGTTATACGAGAGGCCCAAGTTGACAGACACCGGCGTAAAGCGTGGTTAAGGAAAACT-TAGACTAAAGCCGAACACCTTCA
MW232422.1_SRRNA_Scombrinae_Thunnini_Euthynnus-affinis_92%                 --CACCGCGGTTATACGAGAGGCCCAAGTTGACAGACACCGGCGTAAAGCGTGGTTAAGGTAAACT-AAAACTAAAGCCGAATACCTTCA
KP259548.1_SRRNA_Scombrinae_Thunnini_Auxis-rochei_92%                      --CACCGCGGTTATACGAGAGGCCCAAGTTGACAGACACCGGCGTAAAGCGTGGTTAAGGAAAACT-AAAACTAAAGCCGAACACCTTCA
USERDB11988_gb_KM651783_Euthynnus_affinis_92%                              --CACCGCGGTTATACGAGAGGCCCAAGTTGACAGACACCGGCGTAAAGCGTGGTTAAGGTAAACT-AAAACTAAAGCCGAATACCTTCA
MN122853.1_SRRNA_Scombrinae_Scombrini_Scomber-scombrus_95%                 --CACCGCGGTTATACGATAGGCCCAAGTTGACAGACCCCGGCGTAAAGCGTGGTTAGGGAAAATTTAAAACTAAAGCCGAACACCTTCA
USERDB11945_gb_AF055594_Scomber_scombrus_94%                               --CACCGCGGTTATACGATAGGCCCAAGTTGACAGACCCCGGCGTAAAGCGTGGTTAGGGAAAATTTAAAACTAAAGCCGAACACCTCCA
USERDB11943_gb_AB120717_Scomber_scombrus_95%                               --CACCGCGGTTATACGATAGGCCCAAGTTGACAGACCCCGGCGTAAAGCGTGGTTAGGGAAAATTTAAAACTAAAGCCGAACACCTTCA
USERDB11931_gb_475_015_Scomber_australasicus_99%                           --CACCGCGGTTATACGATAGGCCCAAGTTGACAGACCCCGGCGTAAAGCGTGGTTAGGGAAAACTCAAAACTAAAGCCGAATATCTTCA
ANON9_OTU_031_B06-0m_99%                                                   --CACCGAGGTTATACGATAGGCCCAAGTTGACAGACCCCGGCGTAAAGCGTGGTTAGGGAAAACTCAAAACTAAAGCCGAATATCTTCA
AB488406.1_SRRNA_Scombrinae_Scombrini_Scomber-colias_99%                   --CACCGCGGTTATACGATAGGCCCAAGTTGACAGAACCCGGCGTAAAGCGTGGTTAGGGAAAACTCAAAACTAAAGCCGAATATCTTCA
OK501307.1_SRRNA_Scombrinae_Scombrini_Scomber-colias_100%                  --CACCGCGGTTATACGATAGGCCCAAGTTGACAGACCCCGGCGTAAAGCGTGGTTAGGGAAAACTCAAAACTAAAGCCGAATATCTTCA
LC669541.1_SRRNA_Scombrinae_Scombrini_Scomber-japonicus_100%               --CACCGCGGTTATACGATAGGCCCAAGTTGACAGACCCCGGCGTAAAGCGTGGTTAGGGAAAACTCAAAACTAAAGCCGAATATCTTCA
MT410945.1_SRRNA_Scombrinae_Scombrini_Scomber-japonicus_100%               --CACCGCGGTTATACGATAGGCCCAAGTTGACAGACCCCGGCGTAAAGCGTGGTTAGGGAAAACTCAAAACTAAAGCCGAATATCTTCA
AB488405.1_SRRNA_Scombrinae_Scombrini_Scomber-japonicus_100%               --CACCGCGGTTATACGATAGGCCCAAGTTGACAGACCCCGGCGTAAAGCGTGGTTAGGGAAAACTCAAAACTAAAGCCGAATATCTTCA
USERDB11924_gb_475_005_Scomber_japonicus_100%                              --CACCGCGGTTATACGATAGGCCCAAGTTGACAGACCCCGGCGTAAAGCGTGGTTAGGGAAAACTCAAAACTAAAGCCGAATATCTTCA
AB102724.1_SRRNA_Scombrinae_Scombrini_Scomber-japonicus_100%               --CACCGCGGTTATACGATAGGCCCAAGTTGACAGACCCCGGCGTAAAGCGTGGTTAGGGAAAACTCAAAACTAAAGCCGAATATCTTCA
ANON14_OTU_005_C00-300m_100%                                               --CACCGCGGTTATACGATAGGCCCAAGTTGACAGACCCCGGCGTAAAGCGTGGTTAGGGAAAACTCAAAACTAAAGCCGAATATCTTCA
ANON8_OTU_023_B06-0m_99%                                                   --CACAGCGGTTATACGATAGGCCCAAGTTGACAGACCCCGGCGTAAAGCGTGGTTAGGGAAAACTCAAAACTAAAGCCGAATATCTTCA
ANON1_OTU_006_B06-0m_100%                                                  --CACCGCGGTTATACGATAGGCCCAAGTTGACAGACCCCGGCGTAAAGCGTGGTTAGGGAAAACTCAAAACTAAAGCCGAATATCTTCA

                                                                           91
Used4treeSearch                                                            111111111111111111111111111111111111111111111111111111100000111111111111111111111111110000
AP009617.1_SRRNA_Engraulidae_Engraulinae_Amazonsprattus-scintilla_BlueHit  AAACTGTTATACGCATCCAGAGGTCTAAACCCCTCATTCGAAAGAAACTTTATTTTTGTCCACCAGAACCCACGAGAGCTGGGACACAAA
MH613715.1_SRRNA_Engraulidae_Engraulinae_Engraulis-mordax_BlueHit          AAACTGTTATACGCACCCAGAGGTTGAAATCCCTCACACGAAAGTGACTTTATATTCGCCTACCAGAACCCACGAAAGCTGGGACACAAA
LC557053.1_SRRNA_Engraulidae_Engraulinae_Engraulis-japonicus_BlueHit       AAACTGTTATACGCACCCAGAGGTTGAAACCCCTTACACGAAAGTGACTTTATTTTCGCCTACCAGAAGCCACGAAAGCTAGGACA----
DQ912050.1_SRRNA_Engraulidae_Engraulinae_Engraulis-eurystole_BlueHit       AAACTGTTATACGCACCCAGAGGTTGAAACCCCTTACACGAAAGTGACTTTATCTTCGCCTACCAGAACCCACGAAAGCTAGGATACAAA
MT410920.1_SRRNA_Engraulidae_Engraulinae_Engraulis-encrasicolus_BlueHit    AAACTGTTATACGCACCCAGAGGTTAAAACCCCTTACACGAAAGTGACTTTATTTTCGCCTACCAGAACCCACGAAAGCTAGGATACAAA
AF417342.1_SRRNA_Engraulidae_Engraulinae_Engraulis-japonicus_BlueHit       AAACTGTTATACGCACCCAGAGGTTGAAACCCCTTACACGAAAGTGACTTTATTTTCGCCTACCAGAAGCCACGAAAGCTGGGACACAAA
USERDB14368_gb_095_027_Engraulis_japonicus_BlueHit                         AAACTGTTATACGCACCCAGAGGTTGAAACCCCTTACACGAAAGTGACTTTATTTTCGCCTACCAGAAGCCACGAAAGCTAGGACA----
LC604998.1_SRRNA_Engraulidae_Engraulinae_Engraulis-japonicus_BlueHit       AAACTGTTATACGCACCCAGAGGTTGAAACCCCTTACACGAAAGTGACTTTATTTTCGCCTACCAGAAGCCACGAAAGCTGGGACACAAA
ANON2_OTU_321_B06-0m_BlueHit                                               AAACTGTTATACGCACCCAGAGGTTGAAACCCCTTACACGAAAGTGACTTTATTTTCGCCTACCAGAAGCCACGAAAGCTAGGACACAAA
USERDB1672_gb_095_001_Engraulis_japonicus_BlueHit                          AAACTGTTATACGCACCCAGAGGTTGAAACCCCTTACACGAAAGTGACTTTATTTTCGCCTACCAGAAGCCACGAAAGCTGGGACA----
USERDB1697_gb_AB040676_Engraulis_japonicus_BlueHit                         AAACTGTTATACGCACCCAGAGGTTGAAACCCCTTACACGAAAGTGACTTTATTTTCGCCTACCAGAAGCCACGAAAGCTGGGACA----
LC519335.1_SRRNA_Engraulidae_Engraulinae_Engraulis-japonicus_BlueHit       AAACTGTTATACGCACCCAGAGGTTGAAACCCCTTACACGAAAGTGACTTTATTTTCGCCTACCAGAAGCCACGAAAGCTGGGACA----
ANON4_OTU_032_B06-0m_BlueHit                                               AAACTGTTATACGCACCCAGAGGTTGAAACCCCTTACACGAAAGTGACTTTATTTTCGCCTACCAGAAGCCACGAAAGCTGGGACACAAA
USERDB1698_gb_AF417342_Engraulis_japonicus_BlueHit                         AAACTGTTATACGCACCCAGAGGTTGAAACCCCTTACACGAAAGTGACTTTATTTTCGCCTACCAGAAGCCACGAAAGCTGGGACA----
USERDB1716_gb_KF765500_Engraulis_japonicus_BlueHit                         AAACTGTTATACGCACCCAGAGGTTGAAACCCCTTACACGAAAGTGACTTTATTTTCGCCTACCAGAAGCCACGAAAGCTGGGACA----
KF765500.2_SRRNA_Engraulidae_Engraulinae_Engraulis-japonicus_BlueHit       AAACTGTTATACGCACCCAGAGGTTGAAACCCCTTACACGAAAGTGACTTTATTTTCGCCTACCAGAAGCCACGAAAGCTGGGACACAAA
ANON12_OTU_157_B06-0m_BlueHit                                              AAACTGTTATACGCACCCAGAGGTTGAAACCCCTTACACGAAAGTGACTTTATTTTCGCCTACCAGAAGCCACGAAAGCTGGGACACAAA
ANON11_OTU_040_B06-0m_BlueHit                                              AGGCCGTCATACGTACCTAGAGGCTCGAATAACAAACACGAAAGTAGCTTTACCCCTTCCTGCCAGAACCCACGAGAGCTGGGATACAAA
ANON3_OTU_007_B06-0m_BlueHit                                               AGGCCGTCATACGTACCTAGAGGCTCGAATAACAAACACGAAAGTAGCTTTACCCCTTCCTGCCAGAACCCACGAGAGCTGGGATACAAA
ANON10_OTU_391_B06-0m_90%                                                  AGGCAGTTATACGCATCCGAAGATACGAAGCCCCACCACGAAAGTGGCTTTATAA-----CCCCTGACCCCACGAAAGCTATGATACAAA
MW232422.1_SRRNA_Scombrinae_Thunnini_Euthynnus-affinis_92%                 GGGCAGTTATACGCATCCGAAGGCACGAAGCCCCACCACGAAAGTGGCTTTATGA-----CCCCTGACCCCACGAAAGCTATGACACAAA
KP259548.1_SRRNA_Scombrinae_Thunnini_Auxis-rochei_92%                      GGGCAGTTATACGCATCCGAAGGCACGAAGCCCCACCACGAAAGTGGCTTTATGA----CCCCCTGACCCCACGAAAGCTATGACACAAA
USERDB11988_gb_KM651783_Euthynnus_affinis_92%                              GGGCAGTTATACGCATCCGAAGGCACGAAGCCCCACCACGAAAGTGGCTTTATGA-----CCCCTGACCCCACGAAAGCTATGACA----
MN122853.1_SRRNA_Scombrinae_Scombrini_Scomber-scombrus_95%                 GGGCAGTCATACGCTTCCGAAGGCACGAAGCCCCTCCACGAAAGTGGCTTTACTA-----CCCCCGACCCCACGAAAGCTAGGACACAAA
USERDB11945_gb_AF055594_Scomber_scombrus_94%                               GGGCAGTCATACGCTTCCGAAGGCACGAAGCCCCTCCACGAAAGTGGCTTTACTA-----CCCCCGACCCCACGAAAGCTAGGACA----
USERDB11943_gb_AB120717_Scomber_scombrus_95%                               GGGCAGTCATACGCTTCCGAAGGCACGAAGCCCCTCCACGAAAGTGGCTTTACTA-----CCCCCGACCCCACGAAAGCTAGGACA----
USERDB11931_gb_475_015_Scomber_australasicus_99%                           GGGCAGTTATACGCTTCCGAAGACACGAAGCCCTTCCACGAAAGTGACTTTATTG-----CCCCCGACCCCACGAAAGCTAGGACA----
ANON9_OTU_031_B06-0m_99%                                                   GGGCAGTTATACGCTTCCGAAGACACGAAGCCCTTCCACGAAAGTGACTTTATTA-----CCCCCGACCCCACGAAAGCTAGGACACAAA
AB488406.1_SRRNA_Scombrinae_Scombrini_Scomber-colias_99%                   GGGCAGTTATACGCTTCCGAAGACACGAAGCCCTTCCACGAAAGTGACTTTATTA-----CCCCCGACCCCACGAAAGCTAGGACACAAA
OK501307.1_SRRNA_Scombrinae_Scombrini_Scomber-colias_100%                  GGGCAGTTATACGCTTCCGAAGACACGAAGCCCTTCCACGAAAGTGACTTTATTA-----CCCCCGACCCCACGAAAGCTAGGACACAAA
LC669541.1_SRRNA_Scombrinae_Scombrini_Scomber-japonicus_100%               GGGCAGTTATACGCTTCCGAAGACACGAAGCCCTTCCACGAAAGTGACTTTATTA-----CCCCCGACCCCACGAAAGCTAGGACACAAA
MT410945.1_SRRNA_Scombrinae_Scombrini_Scomber-japonicus_100%               GGGCAGTTATACGCTTCCGAAGACACGAAGCCCTTCCACGAAAGTGACTTTATTA-----CCCCCGACCCCACGAAAGCTAGGACACAAA
AB488405.1_SRRNA_Scombrinae_Scombrini_Scomber-japonicus_100%               GGGCAGTTATACGCTTCCGAAGACACGAAGCCCTTCCACGAAAGTGACTTTATTA-----CCCCCGACCCCACGAAAGCTAGGACACAAA
USERDB11924_gb_475_005_Scomber_japonicus_100%                              GGGCAGTTATACGCTTCCGAAGACACGAAGCCCTTCCACGAAAGTGACTTTATTA-----CCCCCGACCCCACGAAAGCTAGGACA----
AB102724.1_SRRNA_Scombrinae_Scombrini_Scomber-japonicus_100%               GGGCAGTTATACGCTTCCGAAGACACGAAGCCCTTCCACGAAAGTGACTTTATTA-----CCCCCGACCCCACGAAAGCTAGGACACAAA
ANON14_OTU_005_C00-300m_100%                                               GGGCAGTTATACGCTTCCGAAGACACGAAGCCCTTCCACGAAAGTGACTTTATTA-----CCCCCGACCCCACGAAAGCTAGGACA----
ANON8_OTU_023_B06-0m_99%                                                   GGGCAGTTATACGCTTCCGAAGACACGAAGCCCTTCCACGAAAGTGACTTTATTA-----CCCCCGACCCCACGAAAGCTAGGACACAAA
ANON1_OTU_006_B06-0m_100%                                                  GGGCAGTTATACGCTTCCGAAGACACGAAGCCCTTCCACGAAAGTGACTTTATTA-----CCCCCGACCCCACGAAAGCTAGGACACAAA

Tree identification:


  
    

        Num_UPLOAD
        UploadedName
        Species
        Genus
        Middle_grouping
        Large_grouping
    

    

        ANON2
        OTU_321_B06-0m_BlueHit
        Engraulis-japonicus
        Engraulis
        Engraulinae
        Engraulidae
    

    

        ANON4
        OTU_032_B06-0m_BlueHit
        Engraulis-japonicus
        Engraulis
        Engraulinae
        Engraulidae
    

    

        ANON12
        OTU_157_B06-0m_BlueHit
        Engraulis-japonicus
        Engraulis
        Engraulinae
        Engraulidae
    

    

        ANON11
        OTU_040_B06-0m_BlueHit
        Auxis-rochei/
Euthynnus-affinis/
Scomber-colias/
Scomber-japonicus/
Scomber-scombrus		
        Auxis/
Euthynnus/
Scomber		
        Scombrini/
Thunnini		
        Scombrinae
    

    

        ANON3
        OTU_007_B06-0m_BlueHit
        Auxis-rochei/
Euthynnus-affinis/
Scomber-colias/
Scomber-japonicus/
Scomber-scombrus		
        Auxis/
Euthynnus/
Scomber		
        Scombrini/
Thunnini		
        Scombrinae
    

    

        ANON10
        OTU_391_B06-0m_90%
        Auxis-rochei/
Euthynnus-affinis/
Scomber-colias/
Scomber-japonicus/
Scomber-scombrus		
        Auxis/
Euthynnus/
Scomber		
        Scombrini/
Thunnini		
        Scombrinae
    

    

        ANON9
        OTU_031_B06-0m_99%
        Scomber-colias/
Scomber-japonicus		
        Scomber
        Scombrini
        Scombrinae
    

    

        ANON14
        OTU_005_C00-300m_100%
        Scomber-japonicus
        Scomber
        Scombrini
        Scombrinae
    

    

        ANON8
        OTU_023_B06-0m_99%
        Scomber-japonicus
        Scomber
        Scombrini
        Scombrinae
    

    

        ANON1
        OTU_006_B06-0m_100%
        Scomber-japonicus
        Scomber
        Scombrini
        Scombrinae
    

  




################ Settings ################

> Number of queries
First2seqs

# Database anonymous
>Number of hits for each query
5
>E-value threshold for reported sequences
1e-3

# Database installed
>Number of hits (SpeciesDB) 
5
>Number of hits (HaplotypeDB) 
5
>E-value
1e-3

# Database user's reference
>Number of hits
5
>E-value
1e-3

>Database installed
MIDORI ver.GB254 (Leray et al. 2022)

>TreeSearchMethod
Neighbor-joining method (Saitou and Nei 1986)

>SubstitutionModel
TN93 (Tamura and Nei 1993) + gamma

>Dependencies
BLAST 2.7.1+
MAFFT v7.356b
trimAl 1.2rev59
ape in R, Version: 5.0

Analysis time: 5.7 seconds